FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18158185
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACCT76000.019.9366421
ACTGAAC77400.019.2064041
CGGACTG70300.019.127031
GGAAAGC61100.019.0175421
GGAACTT57550.018.9478531
TTGAACG69750.018.6468261
GACTAAC61150.018.498151
CGGACGT59150.018.4353141
TGCCCAT72400.018.4202581
ACGCCGA52900.018.3253331
TCTGGAA48900.018.2941971
GGCGACT49750.018.2470441
CTCGAAA54200.018.2309251
TCCCGAT73650.018.2122081
GAATGAA57400.018.1922321
AAACCTG65000.018.1304171
GCCGAAG33750.018.121093
GCGCGAT39550.018.111991
TTTATGC61850.018.0930981
AGCGCCT60850.018.059728
Produced by FastQC (version 0.11.5)