FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12173809
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACCT53100.019.8105951
ACTGAAC52200.019.3722081
CGGACTG47450.019.3403341
GGAAAGC41100.019.0353951
CGGACGT42300.018.703491
TTGAACG47600.018.6090011
AAACCTG44150.018.5677681
GGAACTT38900.018.4995981
CTCGAAA37150.018.3047491
GACTAAC39850.018.2518651
ACGCCGA36000.018.2170431
TGCCCAT51250.018.1854321
GCCGAAG24100.018.1662943
GGACCTA34300.018.1270732
TGACTAG37650.018.032431
CTGAAAC33050.018.0119151
AGCGCCT40350.017.9385058
TCCCGAT50750.017.9309621
GGCGACT32450.017.8702261
TCTGGAA32700.017.8682041
Produced by FastQC (version 0.11.5)