FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16678926
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACCT72300.019.662461
CGGACTG67400.019.31251
ACTGAAC69450.019.2494221
GGAAAGC59550.018.7356991
GACTAAC52650.018.6414681
CGGACGT55900.018.5418381
ACGCCGA48300.018.5206851
GGCGACT45200.018.5007291
TGCCCAT67300.018.4910351
AAACCTG60550.018.4807871
GGAACTT52750.018.3975411
TTGAACG61600.018.3265341
CTCGAAA48550.018.3121
GGACCTA42650.018.2918572
GCCGAAG30750.018.2434063
GCGCGAT33650.018.14771
GAATGAA49650.018.0614241
CGCTATC44600.017.9108181
TGACTAG53050.017.8786851
AGAAATG32050.017.84684210
Produced by FastQC (version 0.11.5)