FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10974942
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGATAG48600.019.6242261
AAACAAG37650.019.5456910
CGGAGCT46200.019.4769541
AGTGCCA61650.019.430538
CCTCTGA55650.019.4113621
ACGCCGA41550.019.3533441
CAATGGA64750.019.2477558
AAGGAGC58600.019.1475071
AGTGTCA36950.019.083291
CATGACA58900.019.0126291
CGGCTAG45500.018.9787641
CTGCGGA36700.018.9135461
CACGAGA24200.018.8635168
GGATTAC47950.018.8578821
TAGAAAG26150.018.8450039
CCGAGCC29550.018.7985279
ATCTGAG47850.018.7820013
CCGTGGA43050.018.7812211
ACGCACC34050.018.76933710
ACTGAAC22700.018.7540381
Produced by FastQC (version 0.11.5)