FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9085811
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGCCA50200.019.8525478
CGGAGCT40100.019.668611
CCTCTGA45450.019.604241
CCGTGGA35950.019.583031
AGTGTCA30250.019.5639151
CTGATAG41300.019.5499151
ATGACAA21900.019.4887562
AAGGAGC48250.019.4241121
TAGAAAG22700.019.3347599
CGGCTAG36300.019.2731
CAATGGA52350.019.2263188
AAACAAG33700.019.19283510
ACGCCGA31400.019.1626931
CACGAGA19100.019.0627868
GTGCCAT45700.019.0152749
TGCACCT30150.018.8626231
CCGAGCC26200.018.8511039
TGACAAC34500.018.8437481
CATGACA50500.018.7765061
GAGTGCC40250.018.7477847
Produced by FastQC (version 0.11.5)