FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9212909
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC17600.019.8763431
CGGAGCT35800.019.6046771
AAACAAG30750.019.49577110
CACGAGA18200.019.4613768
AGTGTCA31450.019.4130761
AGTGCCA54050.019.374498
CTGATAG42500.019.361311
AAGGAGC52500.019.2774931
TGAGCCG30600.019.2333261
CAATGGA53550.019.2061888
CTCGAAA33600.019.1858221
CCTCTGA48050.019.1167961
ACGCCGA33200.019.0193581
ATCTGAG41250.018.9335863
TAGAAAG22150.018.9208359
GGAATAA41150.018.8736931
GTGAGCC22700.018.8513932
ATGACAA20600.018.7972852
CTACAAG21600.018.7919163
CGAACTG27750.018.789039
Produced by FastQC (version 0.11.5)