FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13575423
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGCCA78900.019.8807988
AGTGTCA46900.019.8424471
ACTGAAC25750.019.736151
CTGATAG63900.019.6074261
CGGAGCT56100.019.5688531
CCTCTGA72400.019.4933191
CACGAGA31050.019.3429348
AAACAAG48750.019.33737610
CAATGGA77950.019.2199528
AAGGAGC73050.019.2144581
GTGCCAT72000.019.0818849
GGATTAC65000.018.970991
GAGTGCC63300.018.9415577
TGACAAC50450.018.8386731
GACTAAG41050.018.8379449
ATCTGAG59100.018.8174763
GGAATAA64900.018.8137761
ACGCCGA49000.018.8124621
TGAGCCG43000.018.8025421
ATGACAA30550.018.7596282
Produced by FastQC (version 0.11.5)