FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13756813
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGCCA78400.019.7128568
CTGATAG60000.019.544531
CACGAGA29400.019.4556318
CGGAGCT56500.019.431281
AAGGAGC78300.019.416261
ACTGAAC23750.019.3611871
CCTCTGA74400.019.3546811
AGTGTCA47750.019.3519731
CAATGGA85850.019.309118
CGGCTAG53600.019.189611
TGAGCCG42400.019.14741
GGAATAA68250.019.116191
TAGAAAG35050.019.049789
ACGCCGA47950.018.995951
GAGTGCC61550.018.9795447
GTGCCAT72050.018.9311529
CATGACA76450.018.8500751
CGCTATC45550.018.8375991
GGATTAC62150.018.7976111
ATTCGAG53900.018.7757743
Produced by FastQC (version 0.11.5)