FastQCFastQC Report
Tue 14 May 2024
EGAF00005396597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804448076
Total Bases6.4 Gbp
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGAATT22055166327.416519422441876No Hit
ATGCTCCG21135352426.27310951515036No Hit
CACTCGGA20501167225.48476130608584No Hit
TGAAGTAC15878229619.738041613514902No Hit
GCTGAATA12430090.15451699582410336No Hit
ATGCTCCA10583510.13156237569272278No Hit
CACTCGAA10394580.12921380894694315RNA PCR Primer, Index 47 (100% over 8bp)
TGAAGTAA8945550.11120108639553761No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)