FastQCFastQC Report
Thu 23 May 2024
EGAF00005396608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164934480
Total Bases24.9 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT152774449.262735117605487Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33488632.0304202007973107Clontech SMART CDS Primer II A (100% over 24bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16978341.0293990680420493Clontech SMART CDS Primer II A (100% over 21bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9896510.6000267500161276Clontech SMART CDS Primer II A (100% over 25bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7293640.44221438719181094Clontech SMART CDS Primer II A (100% over 22bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6404150.3882844872703391Clontech SMART CDS Primer II A (100% over 23bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6273570.3803674040746362No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2422870.14689893829355755No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA2222030.13472198172268163Clontech SMART CDS Primer II A (100% over 21bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)