FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005396616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116417246
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGAATT3102232126.647530383943284No Hit
CACTCGGA3019453825.93648195388508No Hit
ATGCTCCG2929781325.166213775577546No Hit
TGAAGTAC2469253421.210374620956074No Hit
CACTCGAA1971700.16936494099851837RNA PCR Primer, Index 47 (100% over 8bp)
ATGCTCCA1485340.12758762563409204No Hit
GCTGAATA1245630.10699703375563446No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)