FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005396628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116961225
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGAATT3306185528.267363820787615No Hit
CACTCGGA3097469626.482875841972415No Hit
ATGCTCCG2953490725.251878988100547No Hit
TGAAGTAC2200472918.813695735488405No Hit
GCTGAATA1675040.14321327431377365No Hit
CAGTCGGA1524770.13036542666169923No Hit
CACTCGAA1491810.12754739872124288RNA PCR Primer, Index 47 (100% over 8bp)
ATGCTCCA1343610.11487653279965221No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)