FastQCFastQC Report
Wed 1 May 2024
EGAF00005396648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218877418
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGAGAT7222943332.999947486588134No Hit
ATGCCTTA5398742824.665599810758003No Hit
TAATGACC5058140623.10946760163262No Hit
CGTATCGG3998334118.267458272008675No Hit
CGTATCGA2922000.13349938183207188No Hit
ATGCCTAA2601850.11887247317583031Illumina PCR Primer Index 3 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)