FastQCFastQC Report
Mon 6 May 2024
EGAF00005396677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165456729
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGAGAT5339375132.27052252435137No Hit
ATGCCTTA4034616724.384724177642845No Hit
TAATGACC3953544623.894734435370108No Hit
CGTATCGG3051524818.443038360803083No Hit
CGTATCGA2311920.13972958452478532No Hit
ATGCCTAA1925730.116388738713673Illumina PCR Primer Index 3 (100% over 8bp)
TAATGACA1662260.10046493787508635No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)