FastQCFastQC Report
Mon 6 May 2024
EGAF00005396681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152240026
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACGCC5375513235.30946060137956No Hit
AGCCAATT5125535633.667464034721064No Hit
CTAGGTGA4499424729.55480774812795RNA PCR Primer, Index 38 (100% over 8bp)
TCGTTCAG7213780.473842535996414No Hit
GATACGCA2439960.1602705979569394No Hit
AGCCAATA2397800.15750128681664832No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)