FastQCFastQC Report
Wed 8 May 2024
EGAF00005396734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97321663
Total Bases778.5 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTCCTC2937036730.17865303020973ABI Solid3 Adapter A (100% over 8bp)
CCGGGTAT2550896426.21098244077477No Hit
TAACAAGG2344751624.09280244214487No Hit
ATCATGCA1782954818.320225374693813No Hit
ATCATGAA1051840.1080787121362692No Hit
GTCATGCA1039510.10681177940824953No Hit
GGTTCCTA1026030.10542668182725157No Hit
GGGTCCTC1002200.10297810056944877No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)