FastQCFastQC Report
Wed 8 May 2024
EGAF00005396775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68221564
Total Bases545.7 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGCAGC2728025939.98773613574734No Hit
CATCACTT1861701627.289048958185713No Hit
ACAATTCA1378381720.204486956646143No Hit
GTGTGGAG64237139.415956808026271No Hit
GTGGGGAG8306171.217528522213299No Hit
GGGTGGAG4851760.7111768941562231No Hit
ACAATTAA1323260.19396506359777974No Hit
CATCACTA1293730.18963652020642624No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)