FastQCFastQC Report
Sun 5 Jun 2022
EGAF00005396786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279372728
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGCTT10273256336.77258110891912No Hit
GATTCTGG6067689121.718974301600404No Hit
CCACTACA5942185421.269740402148344No Hit
ATGAAGAC5387888619.285664132541957No Hit
TGCGGCTA4112570.14720728216535153No Hit
GATTCTGA3272960.11715388339551883RNA PCR Primer, Index 40 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)