FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005396800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43615229
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC33962207.786775577860659No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC21785984.995039691296817No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC14145493.2432456103807223No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC8755742.0074960514365294No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC2875680.659329336548938No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT2128550.48802907810022045No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTA1241440.2846345252480504No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC1224700.2807964163159616No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTGGGTATG1101620.2525769152788353No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCTGGTTTT1097410.2516116561029635No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA1072770.24596225323040263No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG1070400.245418865048261No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG1030020.23616063095759512No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATG963130.22082424466921863No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT905280.20756052891525573No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTTTA903360.2071203157044068No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT898580.20602436823156425No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATAGGGCTGG851780.19529417121712234No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATG842160.19308851960859819No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA773140.1772637717894362No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAAAG752880.1726186053041244No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTAAA745110.17083711746647026No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGA717650.16454115144047507No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCTGGCTTT684020.15683054191919982No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGGATG653970.1499407466139866No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTCTT649300.14887001968968222No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTT615090.1410264290942964No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGTAAA611190.14013224600975957No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTTT607380.13925869791948128No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTTTC606280.1390064924340991No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGCTTT593410.1360556882551276No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTC593130.13599149049521211No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG593120.13598919771807227No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTAAA588840.1350078891022216No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATGG572190.1311904151643913No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTG569080.1304773614739017No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATACGGATGA551190.1263755831707315No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATAGGGAATA544550.12485317914987905No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGCCTG533680.12236093039887513No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATGGGGTTTG507380.11633092652110115No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCGAGCGTG505550.11591134830451079No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGCTTC501340.11494608912863899No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTCTT489490.11222914821793094No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTGGAACTC488920.11209845992096018No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGATGG486120.11145648232180554No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTGC474130.10870744253113976No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG472340.10829703542310876No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAAAG453290.10392929497171734No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGTAAA452550.10375962946336931No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCAGC441010.10111376464399625No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGCTTC438220.10047407982198145No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGACTG437630.10033880597073101No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)