FastQCFastQC Report
Fri 10 May 2024
EGAF00005396814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37198994
Total Bases297.5 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGCTT1340868036.04581349699941No Hit
GATTCTGG827580122.247378517816905No Hit
ATGAAGAC756075520.325159868570637No Hit
CCACTACA754229520.27553487064731No Hit
TGCGGCTA546360.14687493968250862No Hit
GATTCTGA454460.12216997051049283RNA PCR Primer, Index 40 (100% over 8bp)
TGCGGCGT397690.1069088051144609No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)