FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005396829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24070736
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTAT682578828.357205197215407No Hit
GGTTCCTC622170625.84759352601433ABI Solid3 Adapter A (100% over 8bp)
TAACAAGG547268722.735852364464467No Hit
ATCATGCA526163521.859053250386694No Hit
ATCGTGCA445490.1850753545716259No Hit
GGTTCCTA272300.11312491649611378No Hit
TAACAAGA256400.10651938519869106No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)