FastQCFastQC Report
Wed 8 May 2024
EGAF00005396835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11800983
Total Bases94.4 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGATAC378620032.08376793695915No Hit
ACGCGGGT287424824.356004919251216No Hit
GAATTCCA260286222.056315139171033Illumina Small RNA Adapter 2 (100% over 8bp)
TTTACATG243354920.621578727805982No Hit
TTTACATA118940.10078821399878297No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)