FastQCFastQC Report
Wed 8 May 2024
EGAF00005396841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5597149
Total Bases44.7 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGAGAT171401030.62291177168948No Hit
TAATGACC127803322.833642627702066No Hit
ATGCCTTA122014821.799455401312347No Hit
CGTATCGG120515721.531622617157414No Hit
ATGCCTCA1015981.8151741181090586No Hit
AAATGACC104640.18695232161945305No Hit
ATGCCTAA88900.15883086192631285Illumina PCR Primer Index 3 (100% over 8bp)
CGTATCGA66930.11957873553124992No Hit
TAATGACA56080.10019386655599127No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)