FastQCFastQC Report
Wed 8 May 2024
EGAF00005396847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40350587
Total Bases322.8 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGACTC1184390529.352497399851952No Hit
GCCTTGGT1090466127.024789007406508No Hit
AAACCTCA952071323.59498016720302No Hit
TGTAGAAG737178518.269337692658596No Hit
ACACCTCA956530.2370547917927439No Hit
AAATCTCA819690.2031420261618499No Hit
AAACGTCA534690.13251108341992646No Hit
AAACCTAA525320.13018893628486744No Hit
AATCCTCA422700.10475684034038961No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)