FastQCFastQC Report
Thu 9 May 2024
EGAF00005396859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26584471
Total Bases212.6 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTTCG874208932.88419393412041No Hit
CGTGCAGA653986024.600301431613968No Hit
GTATGCTC567404521.343456486307364No Hit
AACAAGAT496333218.67004237172897No Hit
CATGCAGA2346420.8826280575603704No Hit
AACCAGAT1116790.4200911125897521No Hit
TCGCTTCA354430.13332219399814274No Hit
AACACGAT302970.11396502868159385No Hit
GTATGCTA273710.10295860316347841No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)