FastQCFastQC Report
Wed 8 May 2024
EGAF00005396865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48173002
Total Bases385.3 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGGGTG1422162029.521971663713213No Hit
GTGCCCGT1178941624.47307726431498No Hit
CATATAAC1088923422.604433080587338No Hit
AGCTATCA1036864221.523761379869992No Hit
AGCTCTCA3524670.7316691619094031ABI Solid3 EF1 alpha Sense Primer (100% over 8bp)
AGCTTTCA1667100.34606520889024106No Hit
GTGCCCGA525900.10916903206488979No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)