FastQCFastQC Report
Wed 8 May 2024
EGAF00005396874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36244517
Total Bases289.9 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCGCTC1031075228.447756663442362No Hit
TCGATACT980841427.061787028366247No Hit
AGATCTGG889237024.53438681497673No Hit
GTTGAGAA684405618.88301063578803No Hit
CACCGCTA757900.20910749065851808No Hit
AGATCTGA475520.13119777537661767Illumina PCR Primer Index 9 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)