FastQCFastQC Report
Tue 7 May 2024
EGAF00005396886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109586
Total Bases876.6 kbp
Sequences flagged as poor quality0
Sequence length8
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCCAA3102228.308360557005457No Hit
ATCCCCAA1392712.70874016753965No Hit
ATACCTAA95498.713704305294472No Hit
ATATCCAA94238.598726114649681No Hit
ATACACAA88648.088624459328745No Hit
GGGTGTTG60275.499790119175807No Hit
AAACCCAA49464.5133502454693115No Hit
TATGAACT30552.787764860474878No Hit
ACACCCAA27472.506707061120946No Hit
ATTCCCAA24842.2667129012830105No Hit
ATACCAAA22692.0705199569288046No Hit
CCGATAAC20761.8944025696713083No Hit
CCGATACC14781.348712426769843No Hit
ATAGCCAA13561.2373843374153632No Hit
AGACCCAA11871.0831675578997317No Hit
ATACCCAA11471.0466665449966237No Hit
ATACGCAA9820.8960998667713028No Hit
ATACTCAA7840.715419852900918No Hit
ATAACCAA7820.7135948022557627No Hit
ATACCGAA6200.5657656999981749No Hit
GTACCCAA4340.39603598999872247No Hit
CCGATAGA4110.3750479075794353No Hit
CCGATATC3350.30569598306353No Hit
GGTTGTTG3340.30478345774095233No Hit
ATGCCCAA2410.21991860274122604No Hit
AATGAGCT2060.18798021645100652No Hit
ATACCCCA1930.1761173872574964No Hit
CCTATAGC1820.16607960870914168No Hit
TATGACCT1590.14509152628985456No Hit
TATAAGCT1420.12957859580603362No Hit
TATGAGCT1230.1122406146770573No Hit
ATACCCAT1210.11041556403190188No Hit
GGCTGTTC1200.1095030387093242No Hit
GGCTGTTA1170.10676546274159109No Hit
CATGAGCT1140.10402788677385798No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)