FastQCFastQC Report
Wed 8 May 2024
EGAF00005396889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27282443
Total Bases218.2 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAACC764331328.015500664658223No Hit
AGCGCCTA737181827.020373505407857No Hit
CAAAGTGG601224222.03703678589194ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
TTGTTGAT591942021.69681065584926No Hit
GTGTTGAT939880.3444999408594018No Hit
GCTCAACA374360.137216450887481No Hit
AGCGCCAA316480.116001341961935No Hit
CAAAGTGA283120.10377369797858645No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)