FastQCFastQC Report
Thu 9 May 2024
EGAF00005396892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40385165
Total Bases323 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGCTC1205397729.84753683685581No Hit
GTCCTTCT1001894724.80848351121012No Hit
AAGACGGA971710524.061075397364355No Hit
TCTTAAAG815681920.197562644599817No Hit
GGCCTTCT561800.1391104877248861TruSeq Adapter, Index 20 (100% over 8bp)
AAGACGAA515130.12755426404720643No Hit
GTCCTTCA466950.11562414069621853No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)