FastQCFastQC Report
Thu 9 May 2024
EGAF00005396910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48633797
Total Bases389 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGGACGT1447203929.757164549582672No Hit
ATCCCACA1204985224.776704150819235No Hit
GGAATGTC1115782822.942539320958222No Hit
CCTTGTAG988534120.326072833671613No Hit
AGCCCACA4565880.9388286092488316No Hit
TTCCCACA1123370.23098546058412833No Hit
CCTTGTAA904040.18588719280955998Illumina PCR Primer Index 12 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)