FastQCFastQC Report
Mon 13 May 2024
EGAF00005396921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63793809
Total Bases9.6 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC886932013.903104610041392No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC48868567.660392249034699No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC22431213.516204840504194No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG14496292.2723662730344256No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC12241841.9189699113279157No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC9284181.4553418498650863No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC5182180.8123327453295665No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT4471370.7009097073980957No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGGATG3975560.6231889994215584No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGGA3461110.5425463778154397No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT2481650.3890111029426069No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTG1951290.30587450891982326No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTC1514450.2373976446523204No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1428120.22386498351274184No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG1142800.17913964033719948No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT1139430.1786113759095338No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTTT1133840.17773511533070552No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGACCACGTTTTTCAGGTCC996340.15618129966185276No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCCGG991370.15540222719731314No Hit
GGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAG925010.14499996386796718No Hit
CTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGGATGAT908730.14244799209277503No Hit
GTTGGTACCAGGATACACTCCTATGCCCAGAGATAGGGGAGCAGCTCAGT818760.12834474266930823No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA813660.12754529205177262No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTGG804040.12603730873006813No Hit
GCTCAGTGTCACTTGCTGTCCTCTCGTTTTGATCAGATATCTTGGAGTTT771670.12096314863406259No Hit
GTCACTTGCTGTCCTCTCGTTTTGATCAGATATCTTGGAGTTTGAGTGAC754520.11827479998882022No Hit
GTTTCCTTTGTTTCTCTGTGTCTCACTGAAGTATTCAAAGAGGAACTGAA735650.1153168327039384No Hit
ATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCT668590.1048048408584601No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCC667300.1046026268787305No Hit
GTTTGAGTGACTCCAGCCTTTACTGGGCCTGCTCCCAGGAGACAAAGCAG658400.1032075071736193No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)