FastQCFastQC Report
Thu 9 May 2024
EGAF00005396949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32399196
Total Bases259.1 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGAGAA875902327.034692465825387No Hit
GTAATTGC711539221.96163139356915No Hit
TCGTCACG683218221.087504764007107No Hit
AGTCGCGT593854918.329309776699397No Hit
AGTCGCTT29654059.152711690746894No Hit
TCGTCACA3519781.0863788101408443No Hit
CAGGAGAA1475870.4555267359103603No Hit
AGTCGCTG593890.18330393136916115No Hit
AGTCGCTA364780.11258921363357288No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)