FastQCFastQC Report
Fri 10 May 2024
EGAF00005396967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36345444
Total Bases290.7 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAGAGT1121840730.866061231773646No Hit
AGTCCCAC908900225.007266385299904No Hit
CTATAGTG788794621.702709148359833Clontech Universal Primer Mix Short (100% over 8bp)
TACGTTCA785818421.620822681379266No Hit
CTATAGTA437680.1204222460454741No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)