FastQCFastQC Report
Wed 8 May 2024
EGAF00005396973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22992085
Total Bases183.9 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCGCGC707286630.762177505867783No Hit
GGCGAGTA586345125.50204124593311No Hit
CAAATACG488948621.26595304427589No Hit
ACTTCTAT449073819.53166926792416No Hit
GCCGAGTA2461141.0704292368438966No Hit
CCTTCTAT1882500.818760021111613No Hit
GCTTCTAT307690.1338243138888883No Hit
TTGCGCGA256050.1113644108396433No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)