FastQCFastQC Report
Fri 10 May 2024
EGAF00005396988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79832
Total Bases638.6 kbp
Sequences flagged as poor quality0
Sequence length8
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTTCA3314741.520943982362965No Hit
CAAGTACA975312.216905501553262No Hit
TCTTAGGC59877.49949894779036No Hit
AGCCCTTT59067.39803587533821No Hit
CAAGTCCA52556.582573404148712No Hit
CAAATCCA39634.964174767010722No Hit
GTGAGAAG35524.4493436216053714No Hit
AGCCCTAT23682.9662290810702476No Hit
CAAGTCAA16692.09064034472392No Hit
CAAGTGCA14451.8100511073253833No Hit
CCAGTCCA13801.7286301232588435No Hit
CAAGGCCA7830.9808097003707785No Hit
CAAGTCCC5600.7014730934963422No Hit
CAAGACCA3780.47349433811003105No Hit
AGCCCCTT3780.47349433811003105No Hit
AGCCCTTA3550.444683836055717No Hit
AGCCTTTT3520.44092594448341516No Hit
GTGAGAAC2950.3695260046096803No Hit
CAAGTCCG2670.3344523499348632No Hit
CAACTCCA1950.2442629521996192No Hit
CATGTCCA1670.2091892975248021No Hit
GTGAGAAT1620.20292614490429903No Hit
CAAGCCCA1580.1979156228078966No Hit
AGCTCTTT1430.17912616494638742No Hit
GGGAGAAG1380.17286301232588436No Hit
ATCCCTTT910.11398937769315563No Hit
AGCACTTT840.10522096402445136No Hit
GTGGGAAG830.10396833350035074No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)