FastQCFastQC Report
Wed 8 May 2024
EGAF00005396990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79832
Total Bases12 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8991.1261148411664497Clontech SMART CDS Primer II A (100% over 26bp)
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC1880.23549453853091493No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1540.19290510071149414Clontech SMART CDS Primer II A (100% over 24bp)
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC1140.1427998797474697No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1110.13904198817516786No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC950.11899989978955806No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)