FastQCFastQC Report
Fri 10 May 2024
EGAF00005396991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30303
Total Bases242.4 kbp
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTTGAT595719.65811965811966No Hit
GGTCGAGC595419.648219648219648No Hit
CCCACCCA573518.925518925518926No Hit
TTAGATTG549218.123618123618122No Hit
GGTCGGGC7932.616902616902617No Hit
ACCACCCA7322.415602415602416No Hit
CCTACCCA6952.2935022935022933No Hit
CCCCCCCA6202.0460020460020463No Hit
CCAACCCA3631.1979011979011978No Hit
GGTTGAGC3501.1550011550011552No Hit
TAGTTGAT2800.924000924000924No Hit
ATAGATTG2370.782100782100782No Hit
CCCACGCA1940.6402006402006402No Hit
GTAGATTG1780.5874005874005873No Hit
CCCACCAA1710.5643005643005643No Hit
CCCGCCCA1590.5247005247005246No Hit
CCCACCCC1220.4026004026004026No Hit
CCCTCCCA1210.39930039930039923No Hit
TTAGATTA1210.39930039930039923No Hit
TTAGATTC1200.396000396000396No Hit
GGTGGAGC1140.3762003762003762No Hit
CGCACCCA1010.3333003333003333No Hit
CCCAACCA950.3135003135003135No Hit
CCGACCCA950.3135003135003135No Hit
CCCACACA770.2541002541002541No Hit
GGTCGAGA700.231000231000231No Hit
ATGTTGAT700.231000231000231No Hit
CCCAGCCA600.198000198000198No Hit
TAAGATTG540.1782001782001782No Hit
TTAAATTG530.1749001749001749No Hit
GGTCGACC500.165000165000165No Hit
TTAGATAG480.15840015840015842No Hit
GGGCGAGC480.15840015840015842No Hit
GCCACCCA470.1551001551001551No Hit
AAGGTGAT460.1518001518001518No Hit
TTCGATTG440.1452001452001452No Hit
AATTTGAT410.1353001353001353No Hit
GGTAGAGC410.1353001353001353No Hit
TTGGATTG410.1353001353001353No Hit
CACACCCA390.1287001287001287No Hit
GGTCGAGG340.1122001122001122No Hit
AAGTTGAA340.1122001122001122No Hit
TTACATTG320.1056001056001056No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)