FastQCFastQC Report
Fri 10 May 2024
EGAF00005396994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79925
Total Bases639.4 kbp
Sequences flagged as poor quality0
Sequence length8
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCACTA1361817.03847356897091No Hit
CCGAGAAC1060113.263684704410384No Hit
TGCTCTGT77179.655301845480139No Hit
GGCTCTGT46485.815451986237098No Hit
GTAGTGCG46235.784172661870503No Hit
CCAAGAAC44225.53268689396309No Hit
TATCACTA40475.063497028464186No Hit
CCGATAAC28393.552080075070378No Hit
AATCAATA27353.421958085705349No Hit
CCGAGAAA23642.9577729121050984No Hit
AATCATTA21732.7187988739443227No Hit
TGCTCTAT19462.4347826086956523No Hit
CCGTGAAC16702.089458867688458No Hit
AACCACTA12821.604003753518924No Hit
CCGAAAAC12761.5964967156709415No Hit
AAGCACTA11581.4488583046606194No Hit
CGGAGAAC11491.4375977478886457No Hit
AAACACTA10641.331248045042227No Hit
TACTCTGT10271.2849546449796685No Hit
CGCTCTGT9111.1398185799186737No Hit
ACTCACTA6400.8007507037847982Clontech Universal Primer Mix Short (100% over 8bp)
AATCCCTA6150.7694713794182045No Hit
CCGGGAAC5920.7406944010009383No Hit
CCGAGAAT5600.7006568658116985Illumina Small RNA Adapter 2 (100% over 8bp)
AGTCACTA5200.6506099468251486Illumina PCR Primer Index 10 (100% over 8bp)
AATCACAA4830.60431654676259No Hit
CTGAGAAC4080.5104785736628089No Hit
GCAGTGCG4080.5104785736628089No Hit
ATTCACTA3300.4128870816390366No Hit
CATCACTA3210.4016265248670629No Hit
AATCACGA3120.39036596809508917No Hit
GTGGTGCG2810.351579605880513No Hit
CCGAGATC2650.331560838285893Illumina Single End PCR Primer 1 (100% over 8bp)
AATAACTA2490.3115420706912731No Hit
CTAGTGCG2350.2940256490459806No Hit
AATCACTT2350.2940256490459806No Hit
CCGAGTAC2180.2727557084766969No Hit
AATCACCA1760.22020644354081953No Hit
GTTGTGCG1610.2014388489208633No Hit
GCGAGAAC1340.16765717860494211No Hit
TGCTCTTT1270.1588989677822959No Hit
GGAGTGCG1100.13762902721301218No Hit
GATCACTA1100.13762902721301218No Hit
TTAGTGCG1000.12511729746637473No Hit
CCCAGAAC970.12136377854238348No Hit
AATGACTA940.11761025961839223No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)