FastQCFastQC Report
Sat 28 May 2022
EGAF00005397691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005397691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9131101
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT629240.689117336452636No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA560710.6140661460211644No Hit
GTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGC429340.4701952152319857No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT422780.46301097753710097No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA415390.4549177585485036No Hit
CACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAGC389930.42703503115341734No Hit
CTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGT386800.42360718603375436No Hit
ATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCAC354180.3878831260326657No Hit
CTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTC349840.3831301395089157No Hit
CTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCT345790.37869474885887255No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT345670.37856332987664904No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGACTTCC337920.37007585394138126No Hit
CTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTC322750.3534623042719602No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGC314170.3440658470429798No Hit
CACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACTG309330.33876528142663187No Hit
CTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAAT304330.33328949050065265No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC301320.32999306436321313No Hit
GTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCA292740.32059660713423277No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT287450.3148032203345468No Hit
TGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGC278500.3050015545770439No Hit
CAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGG274410.30052235759959284No Hit
CTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGA269570.29522179198324494No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG266930.2923305743743279No Hit
CTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCATTGAATT261340.28620864011908315No Hit
GTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGC252900.2769655050360302No Hit
CTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCG251370.2752899130126805No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG245970.2693760588126229No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT240750.2636593330859006No Hit
GTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCG226520.24807523211056365No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT224820.24621346319573073No Hit
ATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCC224230.24556731986646518No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAACCCTTCT221950.24307035920421863No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC221580.24266515067569616No Hit
CACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAGGGCA219540.24043102797789662No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA216190.23676224805749055No Hit
CGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGC214370.23476906016043408No Hit
CTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAAC213290.2335862893204226No Hit
GTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAA210270.23027891160113112No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA203580.22295230334217092No Hit
CTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGA199850.21886736331139037No Hit
AGCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCA194930.2134791850402268No Hit
CCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAA192390.21069748324982934No Hit
GATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTAT189260.20726963813016636No Hit
CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTG182040.19936259603305231No Hit
CACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAA177980.19491625380115715No Hit
ATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCG176680.19349254816040257No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT175040.19169648873668135No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG174530.1911379580622315No Hit
CGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCAT171690.18802770881627529No Hit
CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT168360.1843808320595731No Hit
CTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAG168100.18409609093142218No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTC167950.1839318172036428No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA167740.18370183398475168No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT166320.18214670936177357No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG164390.18003305406434558No Hit
GATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCG163430.17898170220655757No Hit
CAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA163150.17867505791470273No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA162060.17748133549283926No Hit
ATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTG159310.17446965048355068No Hit
CAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGC152770.16730731595236983No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT152550.16706638115162672No Hit
CTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGACGGG150930.16529222489160947No Hit
AGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGA150270.1645694204893802No Hit
GTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATC148000.16208341140898563No Hit
CTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAG147920.16199579875416995No Hit
GCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAG147680.16173296078972296No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG147530.16156868706194358No Hit
CCACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAG143770.15745089228560719No Hit
CTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAGGG141030.15445015885817054No Hit
CCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACC138480.15165750548592113No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA138340.15150418333999371No Hit
TGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGC136850.1498723976440519No Hit
CGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCACCA134600.14740829172736122No Hit
CGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAG133270.14595173134105077No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG131620.14414472033547762No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC130160.1425457893850917No Hit
GTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCT129730.14207487136545746No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC129000.1412754058902645No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC128780.1410344710895214No Hit
CTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC127400.13952315279395114No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT127150.13924936324765216No Hit
CCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGC124670.13653337094836646No Hit
CAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACTGAT124140.13595293711021267No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC123710.13548201909057844No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT123320.13505490739835208No Hit
GTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACT122070.13368595966685726No Hit
GTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAG121450.13300696159203582No Hit
CCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTC121200.13273317204573687No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG119150.1304880977660854No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG118930.13024716296534228No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC118650.12994051867348747No Hit
CTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAA118630.12991861550978354No Hit
CGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC117460.1286372804331044No Hit
ATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCC116000.12703834948271847No Hit
TGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTC115670.12667694728160384No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA114190.12505611316751397No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC111260.12184729968489014No Hit
CTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCT111050.12161731646599902No Hit
GTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCAT110200.12068643200858255No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT110150.12063167409932275No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA108650.11898893682152896No Hit
AACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTG108020.11829898716485558No Hit
GGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGC107950.11822232609189187No Hit
CTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAAC107680.11792663338188901No Hit
TATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATC107550.11778426281781353No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG107460.11768569858114591No Hit
CTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCA106940.11711621632484406No Hit
CTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCG104130.11403882182444372No Hit
GTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTT103550.11340363007703014No Hit
CTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACC102780.11256035827442934No Hit
CTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCC101950.11165137698071678No Hit
CATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCC101800.11148710325293741No Hit
CTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAGCGTCGGC99740.10923107739143395No Hit
CTTGTGTCGAGGGCTGACTTTCAATAGATCGCAGCGAGGGAGCTGCTCTG99690.10917631948217416No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC98840.10824543502475768No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC97790.10709551893030204No Hit
CCCGCTTCGCGCCCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCCTTA97610.1068983904569668No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTA97070.10630700503696103No Hit
CTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAA96830.10604416707251405No Hit
CACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCT96770.10597845758140229No Hit
CAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAG96650.1058470385991788No Hit
CCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCT96440.10561705538028765No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT96130.10527755634287694No Hit
GTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCT95730.10483949306879861No Hit
CAACTACGAGCTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCT95270.1043357203036085No Hit
CTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGC93880.1028134504261863No Hit
CAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT93780.10270393460766669No Hit
CTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGA92880.10171829224099042No Hit
TGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTC92140.1009078751839455No Hit
CCCCGCTTCGCGCCCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCCTT91510.10021792552727213No Hit
CTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTAC91430.10013031287245645No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)