FastQCFastQC Report
Sat 28 May 2022
EGAF00005397695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005397695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9288852
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT641910.6910541797845418No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA562530.6055969026097089No Hit
GTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGC455740.49063113504230665No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT428320.46111187905674456No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA418830.45089533130681814No Hit
CACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAGC395970.4262851857258572No Hit
CTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGT392990.42307703901407834No Hit
ATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCAC371400.39983412374317084No Hit
CTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTC355840.38308286104676875No Hit
CTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCT353420.38047758754257255No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT352070.37902423248857875No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGACTTCC344620.3710038657091318No Hit
CTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTC326440.35143201764868254No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGC318350.34272265291771253No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC313940.3379750264079996No Hit
GTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCA310980.3347884108822059No Hit
CACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACTG309210.3328829009225252No Hit
CTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAAT307930.33150490501947927No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT293900.31640077805093675No Hit
TGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGC283910.30564595065138295No Hit
CTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGA275460.29654902457268134No Hit
CAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGG274230.29522485663459813No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG268370.288916219140966No Hit
CTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCATTGAATT264670.2849329497337238No Hit
GTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGC259380.2792379510406668No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG253550.2729616103260123No Hit
CTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCG251930.2712175842612198No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT247160.2660823964037752No Hit
ATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCC239970.2583419350421344No Hit
GTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCG232770.250590708087501No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT230090.2477055291654986No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC227190.24458350719766017No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAACCCTTCT222310.23932989781729758No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA221970.238963867655551No Hit
GTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAA220190.23704759210287774No Hit
CACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAGGGCA217970.23465763045853244No Hit
CGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGC217270.2339040389490542No Hit
CTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAAC215840.2323645591511201No Hit
CTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGA205780.22153437260061848No Hit
AGCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCA204960.22065159397522965No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA204220.21985494009378123No Hit
CCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAA191820.20650560478302377No Hit
GATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTAT191610.20627952733018032No Hit
ATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCG189660.20418023669663377No Hit
CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTG181790.19570771501149978No Hit
CACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAA181750.19566465263952962No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT179240.1929624887984005No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG175240.18865625160138197No Hit
CGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCAT173800.18710600621045528No Hit
ATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTG173230.18649236740988015No Hit
CTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAG173200.1864600706309025No Hit
GCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAG173060.18630935232900686No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA172350.18554499522653606No Hit
CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT171000.18409164017254231No Hit
GATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCG169420.18239067647972No Hit
CAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA168710.1816263193772492No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTC167960.18081889990280822No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA167950.18080813430981568No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG167650.1804851665200393No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT166000.17870884367626913No Hit
GTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATC156570.16855688948429795No Hit
AGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGA155970.16791095390474517No Hit
CTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAG154640.16647913003673653No Hit
CTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGACGGG152590.16427218347326453No Hit
CAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGC151910.16354012314977137No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT151880.16350782637079372No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG149520.1609671464245528No Hit
CCACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAG147580.1588786213839988No Hit
CTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAGGG141870.15273146778525484No Hit
CCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACC140180.15091208256951452No Hit
TGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGC138570.14917882209771455No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA137550.14808073161247484No Hit
CGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCACCA136530.1469826411272351No Hit
CGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAG136190.14661661096548853No Hit
GTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCT134610.1449156472726662No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC134510.14480799134274075No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG133190.14338693306772463No Hit
GTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACT132850.14302090290597805No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC131020.14105079938834209No Hit
CTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC130960.1409862058303868No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC130240.14021108313492345No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT128770.13862854096501914No Hit
GTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAG127160.13689528049321917No Hit
CAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACTGAT126090.13574336204301674No Hit
CCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGC125320.13491441138259067No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC124470.13399933597822422No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT124230.1337409617464031No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC123770.13324574446874599No Hit
CCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTC120480.12970386437419823No Hit
CTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAA120240.1294454901423771No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG119700.12886414812077962No Hit
ATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCC118970.12807825983232374No Hit
CGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC118590.12766916729860697No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA117920.12694787256810638No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC117390.1263772961395014No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG116660.12559140785104556No Hit
TGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTC115440.12427800550595489No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT114610.12338446128757354No Hit
GTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCAT113130.12179115352467668No Hit
GGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGC112480.12109138998016115No Hit
AACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTG112010.12058540710951149No Hit
CTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCT111370.11989640915798852No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA110990.11948731662427177No Hit
CTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAAC110390.11884138104471897No Hit
TATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATC108770.11709735497992647No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG107110.11531026654316377No Hit
CTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCA107030.11522414179922341No Hit
GTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTT105300.1133616942115129No Hit
CTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCG102820.11069182714936139No Hit
CTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACC102420.11026120342965955No Hit
CATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCC102270.11009971953477135No Hit
CTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCC101750.10953990869915894No Hit
TGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAA101730.10951837751317385No Hit
CTTGTGTCGAGGGCTGACTTTCAATAGATCGCAGCGAGGGAGCTGCTCTG101150.10889397311960618No Hit
CTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAGCGTCGGC100940.1086678956667627No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC99840.1074836804375826No Hit
GTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCT98870.10643941791730561No Hit
CTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAA98220.10573965437279009No Hit
CCCGCTTCGCGCCCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCCTTA97610.10508295320024476No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG97570.10503989082827458No Hit
CAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAG96840.1042540025398187No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT96760.10416787779587833No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTA96700.10410328423792305No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC96030.10338198950742244No Hit
CCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCT95490.10280064748582494No Hit
CACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCT95330.1026283979979442No Hit
CAACTACGAGCTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCT94210.10142265158277902No Hit
CTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGA93930.10112121497898771No Hit
CTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGC93750.10092743430512188No Hit
TGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTC93480.10063676329432313No Hit
CAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT93000.10012001483068092No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)