FastQCFastQC Report
Sat 28 May 2022
EGAF00005397700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005397700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9318203
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1703581.8282280392474815No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC831510.8923501666576699No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA615880.6609428878078746No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCG359530.3858361960991835No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG335880.3604557659883564No Hit
CCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAA309560.33220997653732165No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACA273060.293039333871563No Hit
CTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGC260730.2798071688285821No Hit
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG241040.2586764851549167No Hit
CACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGA231340.2482667527204548No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG222520.23880140838313996No Hit
CAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGG221180.23736336287157514No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT218310.23428336987292506No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT210210.2255907067060033No Hit
CGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGG208790.22406680773106144No Hit
CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTT207640.22283266419501702No Hit
CTTGAAGCCTAGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCT181680.19497321532917883No Hit
CTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTT176020.1888990827952557No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA174530.187300062039859No Hit
GTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATT173730.1864415274060889No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG164740.17679374445909796No Hit
CCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATT157010.16849815356029485No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA156320.16775766743866816No Hit
GTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTA154710.16602986648820592No Hit
CGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATG152080.1632074338796869No Hit
CGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCT150200.16118987749032726No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA146030.15671476571180087No Hit
CCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCT145070.15568452415127682No Hit
CGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAGCCAAGGG143230.1537098944936057No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAAC141000.15131672920197167No Hit
CGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCA137930.1480221025448791No Hit
TCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCG133250.1429996749373243No Hit
CATTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCG131070.1406601680603009No Hit
GTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGA128960.13839578296373237No Hit
CTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTTAA122570.1315382375764941No Hit
GTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCA120770.12960653465051147No Hit
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA118370.12703093074920133No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA118180.12682702877368093No Hit
CTTGCGTTGATTAAGTCCCTGCCCTTTGTACACACCGCCCGTCGCTACTA116710.12524947138412848No Hit
CGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGA116490.1250133743598417No Hit
CTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGC114960.12337142687275648No Hit
CACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGT113930.12226606353177753No Hit
GTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTC113800.12212655165378991No Hit
CGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTC113500.12180460116612614No Hit
CAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCA112580.12081728633729057No Hit
CTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGA110160.11822021907013616No Hit
GTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTC109700.11772656165571838No Hit
GTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAAT109010.11698607553409172No Hit
TGTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGGTCTCCAAGGT108490.11642802802214118No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT108420.1163529062416863No Hit
CGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTC108150.11606315080278891No Hit
CAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAA107660.11553729833960476No Hit
AGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGA106110.11387388748667526No Hit
CACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAAGACCCTG104090.1117060875364059No Hit
AATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCT103670.11125535685367662No Hit
CCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTC103490.11106218656107836No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAATTATTCCCCATGA103370.11093340636601284No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATG102500.10999974995178792No Hit
CGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGC102070.10953828758613653No Hit
TGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTA102050.10951682422029226No Hit
CGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAA101930.10938804402522675No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC101870.109323653927694No Hit
CCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACCGGCACGAGACC101340.10875487473282135No Hit
GTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTCT100880.10826121731840356No Hit
CTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAA100700.1080680470258053No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC100480.10783195000151855No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAA100090.10741341436755562No Hit
CTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATA99020.10626512429488819No Hit
CGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTT98970.10621146588027756No Hit
CGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAAG98930.10616853914858905No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA98900.10613634409982267No Hit
CGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA98610.10582512529508104No Hit
GTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC98520.10572854014878191No Hit
CTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCCCCGCCACGCAGT97780.10493439561254461No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT96800.10388269068617628No Hit
CACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACA96340.10338903327175852No Hit
CTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAG94860.10180074419928392No Hit
CAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAG94800.10173635410175116No Hit
CCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGA94660.1015861105408414No Hit
CGAAGGCCCGCGGCGGGTGTTGACGCGATGTGATTTCTGCCCAGTGCTCT94010.10088855115090323No Hit
GACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGT93570.1004163571023297No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)