FastQCFastQC Report
Sat 28 May 2022
EGAF00005397745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005397745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10895239
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC1133381.0402525359930148No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG1073680.9854579601236834No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA956060.8775025495080925No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT720330.6611419905520199No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG666200.6114597394329762No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT665710.6110100017080855No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG595930.5469636783552889No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC548190.503146374301656No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA546190.5013107101184288No Hit
CTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGC408510.3749435877450692No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA387500.3556599355002676No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC346880.3183775959389234No Hit
AGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGA341870.3137792571599393No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC320480.29414682872032455No Hit
CGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC318760.2925681575227491No Hit
CACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCG272990.250558982689595No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT251830.23113765563105132No Hit
CTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACCATCGGGAT250920.23030242842768295No Hit
GTGAGTTTTAGCTTTATTGGGGAGGGGGTGATCTAAAACACTCTTTACGC240820.22103232430238565No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA237920.21837061123670623No Hit
CTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAA230530.2115878320796818No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT223600.20522725568479955No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA219310.20128975601177726No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG213560.1960122214849991No Hit
GTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTATTTCC206260.18931204721621986No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC206050.189119302476981No Hit
ATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAA203570.1868430788897793No Hit
TTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTC203560.18683390056886315No Hit
GATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATT199450.1830616106723313No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC199050.18269447783568585No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC198730.1824007715663695No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC189990.1743789190856667No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG189270.1737180799797049No Hit
GTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCA188990.1734610869940531No Hit
GTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTG185730.17046895437539278No Hit
CGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC182000.16704544067367408No Hit
ACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGG179310.1645764723472335No Hit
GGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATC177640.1630436927542388No Hit
GTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATT173860.15957428744793942No Hit
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA172270.1581149344222738No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG165240.15166257481823023No Hit
CTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGG163440.1500104770533258No Hit
CGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAA162400.14905593167804762No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG160470.1472845157412334No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC160100.14694491786733638No Hit
GTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTGATGCTA157680.14472376420563146No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC152890.14032734848680237No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA152830.14027227856130553No Hit
CACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTG150620.1382438696388395No Hit
GTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTA150250.13790427176494247No Hit
TAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCG146490.13445322310047536No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCA145540.13358128261344243No Hit
GTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTC141590.12995584585156875No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCC141330.12971720950774923No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT138690.12729413278588933No Hit
CTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT137780.12645890558252096No Hit
GTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAG137580.12627533916419825No Hit
GTCTGTTCCAAGCTCCGGCAAAGGAGGCATCCGCCGGGCCCCTCCCCGAA134370.12332909815011861No Hit
GTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGA133040.1221083814682725No Hit
CTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACGGG132800.12188810176628526No Hit
TATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGA132540.12164946542246571No Hit
CTCGTATACCCTTGACCGAAGACCGGTCCTCCTCTATCGGGGATGGTCGT132210.12134658083223324No Hit
CTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACT131620.1208050598981812No Hit
ATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGAT131280.1204929969870326No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT129730.11907035724503152No Hit
GATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTT129380.11874911601296677No Hit
GGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTA129220.11860226287830858No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATG125950.11560095193873213No Hit
TCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA123220.11309527032862703No Hit
GGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCTGAACTCA121400.11142481592189028No Hit
CTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATA117540.10788198404826181No Hit
GTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTT116550.10697333027756435No Hit
AGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCTGAACTC115540.10604631986503461No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAG115380.10589946673037645No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTAC115380.10589946673037645No Hit
GCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGT112380.10314597045553567No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT110460.10138373283963757No Hit
GTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTC110390.10131948459322461No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)