FastQCFastQC Report
Sat 28 May 2022
EGAF00005397762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005397762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11032878
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT525460.47626738916174No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC431740.3913212853436791No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC297120.26930416524138123No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG273700.24807670310502844No Hit
CACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCA265900.24100692493835246No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA258310.23412748695308697No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT245490.22250767206888358No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT236330.21420521463212044No Hit
CTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGG205350.1861255059649894No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC197660.17915542979809984No Hit
GGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGG176850.16029362420213475No Hit
ATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGC176800.1602483051113227No Hit
GATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG171010.15500035439529014No Hit
CTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCG168960.15314227167199712No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT167600.15190959240191No Hit
GTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAAC159140.14424160223651528No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT148460.13456144443906656No Hit
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG148370.1344798700756049No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG145980.1323136175347901No Hit
CCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGG143910.13043740717517224No Hit
CGAGTAGCTGCGCTCCCCTGCTAGAACCTCCAAACAAGCTCTCAAGGTCC141900.12861557972452883No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT141460.12821677172538298No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG141420.12818051645273337No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG132360.11996869719759433No Hit
GGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGA126380.11454853393647606No Hit
GGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT125200.11347900339331225No Hit
GGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACG124620.11295330193989274No Hit
ATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGT121310.10995317812813664No Hit
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG117970.10692586286189334No Hit
GATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG114730.10398918577727406No Hit
CTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCTAGAACCTCCAA112040.10155101869158709No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT111590.10114314687427886No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)