FastQCFastQC Report
Sat 28 May 2022
EGAF00005397804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005397804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18764901
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1543140.8223544584647688No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC915090.4876604464899655No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT714580.38080669863379507No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG615540.32802731013608866No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA595580.31739043014402263No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACA409460.2182052545867415No Hit
CTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTT333690.1778266775827914No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG318270.16960920817008307No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC310100.1652553349468777No Hit
GTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTC303850.16192464857661654No Hit
CCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAA297390.1584820511443146No Hit
CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTT282230.15040313828460913No Hit
CCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATT281680.15011003788402616No Hit
GTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTC262620.1399527767292777No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT256320.13659544486805444No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA240130.12796763489452995No Hit
CGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAA235690.12560151529709643No Hit
CACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGT230060.12260123301476517No Hit
GTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTA228400.1217166027148238No Hit
CGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA226420.12066144127272507No Hit
CCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAG220020.11725081842957764No Hit
CGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATG219220.11682449057418422No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT212890.11345117141838372No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAAC208300.11100511534806391No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATTTAC204500.10898005803494513No Hit
GTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCA200760.10698697531098086No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC197860.10544153683517968No Hit
CGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTC194720.10376820000276048No Hit
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA191590.10210019226853369No Hit
TGTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGGTCTCCAAGGT191390.10199361030468533No Hit
CCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCCC191040.10180709186795071No Hit
CACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGA190350.10143938409267388No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCG190340.10143405499448147No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)