FastQCFastQC Report
Sat 28 May 2022
EGAF00005397879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005397879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22002711
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG1243410.5651167258434654No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA860620.3911427096415528No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA844470.3838027050393926No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT781950.3553880246847763No Hit
CGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGC680180.3091346334549411No Hit
CACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACTG624940.28402863628941No Hit
CTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCG612010.27815208771319133No Hit
GTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCG611800.2780566449288908No Hit
CTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGA559520.25429593653254823No Hit
CTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGACGGG476050.21635970222033094No Hit
CAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACTGAT464700.2112012469736116No Hit
CTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAAT452360.2055928471723325No Hit
GATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTAT449560.2043202767149921No Hit
CTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTC446180.2027841023772025No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGACTTCC444180.20187512347910216No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT438320.1992118153076682No Hit
ATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCAC433510.19702572105773694No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT424520.19293986091077597No Hit
GTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCA423420.19243992251682077No Hit
CTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGT419530.19067195856001562No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT414420.1883495174753693No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC411850.18718147959131035No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA403570.18341830695317501No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGC401120.1823048078030021No Hit
CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTG385560.17523295197578154No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA383930.17449213417382975No Hit
CAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA382570.17387402852312153No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC374270.17010176609600516No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC358710.1630299102687846No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG358010.1627117676544495No Hit
ATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCC348880.15856227898462147No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA348340.15831685468213438No Hit
CTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGA347570.15796689780636577No Hit
CTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCT347370.15787599991655574No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG339570.15433098221396443No Hit
CACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAGC333900.15175402703785001No Hit
CTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCC333430.15154041699679643No Hit
CTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCATTGAATT331680.15074506046095865No Hit
CCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGC325630.14799539929420516No Hit
GGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGC321220.1459911008238939No Hit
TGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGC320490.1456593235260873No Hit
CTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTC318200.14461854268776245No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC316780.1439731676701112No Hit
CAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGG313020.1422642873416826No Hit
CTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAG306760.13941918339062856No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG290280.13192919727028182No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC287260.13055663913415033No Hit
CGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGA285410.12971583365340753No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC277680.12620263021224976No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTC277200.12598447527670567No Hit
CTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAAC272200.12371202803145484No Hit
CCGGAAGCTGCCCGGCGGGTCATGGGAATAACGCCGCCGCATCGCCGGTC270980.12315755090361366No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC268190.1218895253407637No Hit
CTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTC266170.12097145665368235No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT265870.1208351098189673No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT265200.12053060188810369No Hit
CTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCG264470.12019882459029707No Hit
CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT263850.11991704113188596No Hit
GTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCAT262970.11951709041672183No Hit
CCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCAC261880.11902169691725714No Hit
GTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGC261760.11896715818337111No Hit
ATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCG260120.11822179548692886No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA257460.11701285355245543No Hit
CTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTAC256060.11637656832378519No Hit
CCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGG247650.11255431205727331No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA243160.11051365443103807No Hit
CTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGC242760.110331858651418No Hit
CTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAGCGTCGGC242740.110322768862437No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGG234700.10666867369207367No Hit
CTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGC234120.10640506981162458No Hit
CCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAAC231490.10520976256062264No Hit
AGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGA231260.1051052299873411No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG230100.10457802222644291No Hit
GTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATC227860.10355996586057055No Hit
CAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGC226580.10297821936578633No Hit
GATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCG226410.10290095615944782No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT224940.10223285666934408No Hit
CTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGA224860.10219649751342005No Hit
ATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATC224460.10201470173379998No Hit
CTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAA223430.10154657760127832No Hit
GTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGC220400.10016947457065631No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)