FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005398223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005398223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42686755
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC12323072.8868603387631597No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCGTATGCC10685292.503186292797379RNA PCR Primer, Index 40 (100% over 51bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTC9503692.226379119237337RNA PCR Primer, Index 1 (97% over 36bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA7360471.7242983215753924RNA PCR Primer, Index 1 (100% over 28bp)
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACATG6640621.555662874819133No Hit
CGGTTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGC5648531.32325120520405No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA5444541.2754635483535817No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA4919371.1524347540589581No Hit
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTC4805871.125845710220887No Hit
TTAAGTAAGAGTATTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC4492811.0525068021684947RNA PCR Primer, Index 1 (100% over 33bp)
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGA3990760.9348942078169211RNA PCR Primer, Index 40 (100% over 39bp)
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT3866160.9057048257708977No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG3799290.8900395450532607RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG3697330.8661539158926463RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTCC3610130.8457260337544983RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG2828120.6625286930337057RNA PCR Primer, Index 1 (100% over 29bp)
CACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTC2676640.6270422757597761No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG2618710.613471321490706RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG2615430.6127029332634912RNA PCR Primer, Index 1 (100% over 29bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2570980.602289867196511RNA PCR Primer, Index 1 (100% over 32bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC2545800.5963910819644173RNA PCR Primer, Index 2 (100% over 34bp)
CACGGTCCCCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAA2199050.5151597960538346RNA PCR Primer, Index 1 (100% over 23bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG1973900.4624150980790177Illumina Small RNA Adapter 2 (100% over 21bp)
ATCCCACCGCTGCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1970310.46157408779374304RNA PCR Primer, Index 1 (100% over 33bp)
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTCA1857640.43517948365950987No Hit
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG1855440.43466410131198774RNA PCR Primer, Index 1 (100% over 29bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG1792530.41992650881989035No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTGC1770510.41476800005060116No Hit
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAAT1768760.4143580368196177RNA PCR Primer, Index 40 (100% over 41bp)
GCCCCGCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAA1724060.40388640457678265RNA PCR Primer, Index 40 (100% over 40bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1660190.3889239179694029RNA PCR Primer, Index 1 (100% over 33bp)
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA1644420.3852295635964833RNA PCR Primer, Index 1 (100% over 28bp)
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGGT1639080.3839785900802251No Hit
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA1536420.35992897562721743RNA PCR Primer, Index 1 (100% over 28bp)
TCACGGAGAAGATCGCGGGTTCGATTCCCGTCTGGACCGCCATGGAATTCT1475240.3455966610720351No Hit
CGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAA1455610.3409980449439176RNA PCR Primer, Index 40 (100% over 40bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1436660.33655872881412513RNA PCR Primer, Index 1 (100% over 33bp)
CCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1433830.335895759703449RNA PCR Primer, Index 1 (100% over 32bp)
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCTGATGCCATCACCGC1250950.2930534307421588No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTCC1239550.2903828131231807RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG1228180.2877192234453052RNA PCR Primer, Index 1 (100% over 29bp)
CCCCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATC1210850.2836594161350517RNA PCR Primer, Index 40 (100% over 42bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCA1089630.255261848786585RNA PCR Primer, Index 1 (97% over 37bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT1075710.25200088411499066RNA PCR Primer, Index 1 (97% over 35bp)
CCCCGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAAT1065890.24970040472741487RNA PCR Primer, Index 40 (100% over 41bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG1016220.23806447690858673RNA PCR Primer, Index 1 (100% over 29bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT1003590.23510571370440317RNA PCR Primer, Index 1 (97% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGTGGAATTCTCGGGTGCCAA995030.2331004078431354No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG977320.22895157994558263RNA PCR Primer, Index 1 (100% over 29bp)
CCCTGCGGGCCGCCGGTGAAATACCACTACTCTGTGGAATTCTCGGGTGCC944140.22117867708613598No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAGGA905060.2120236124765164RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG901890.21128099336667777No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG892070.20898051397910197RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATGGAATTCT886330.20763583458147614No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCCA870560.20394148020855649No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGTG865250.2026975346334009No Hit
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAAT857480.2008772978878343No Hit
ACCCTGTAGATCCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA852700.19975751260549085RNA PCR Primer, Index 1 (100% over 28bp)
GTCCAGTTTTCCCAGGAATCCCTTGGAATTCTCGGGTGCCAAGGAACTCCA829300.19427571854548326RNA PCR Primer, Index 1 (100% over 28bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCTC818090.1916496112201548No Hit
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCCA782590.18333321424877577RNA PCR Primer, Index 1 (100% over 28bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC780710.1828927966063478No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT776800.181976821615979RNA PCR Primer, Index 1 (100% over 30bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG775860.18175661279476504RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAATGGAATTCTCGG768450.18002071134242928No Hit
CCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT758970.17779988195401594RNA PCR Primer, Index 1 (97% over 35bp)
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCTC754930.17685345255220267No Hit
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT737130.17268354083134219RNA PCR Primer, Index 1 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCAA733520.17183784525199913No Hit
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG732960.1717066570180844No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC726630.17022376144544132RNA PCR Primer, Index 1 (100% over 31bp)
CGCGCCGGCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTC725000.16984190997886814RNA PCR Primer, Index 1 (97% over 36bp)
CTCGGATCGGCCCCGCCGGGGTCTGGAATTCTCGGGTGCCAAGGAACTCCA716200.1677803805887798RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT715940.16771947176589086RNA PCR Primer, Index 1 (97% over 35bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT706700.1655548659062981No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAAC692750.16228687329360125RNA PCR Primer, Index 1 (100% over 24bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCG676110.15838870862870696No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGGA674860.15809587774943307RNA PCR Primer, Index 1 (100% over 22bp)
TCCCCGGCACCTCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC655270.15350663220945232RNA PCR Primer, Index 1 (100% over 33bp)
CCCGCGAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGTGGAATTCTCGGGTG651030.15251334986695522No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC634670.14868077931901827RNA PCR Primer, Index 2 (100% over 34bp)
CCGCCGCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAG634640.1486737513779157RNA PCR Primer, Index 40 (100% over 38bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA609500.1427843367339588No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCTTGATGAAAGCACAT604750.14167157939271793No Hit
CCCCGCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGA593090.1389400529508509RNA PCR Primer, Index 40 (100% over 39bp)
ACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC589490.13809670001854205RNA PCR Primer, Index 1 (100% over 31bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA583340.1366559720925144RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC575340.1347818544651614RNA PCR Primer, Index 1 (100% over 27bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT560320.1312631986198061RNA PCR Primer, Index 1 (97% over 35bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATTCTCGGGTGCCAAGGA556080.13026991627730897RNA PCR Primer, Index 1 (100% over 22bp)
CACGGAGAAGATCGCGGGTTCGATTCCCGTCTGGACCGCCATGGAATTCTC555340.1300965603967788No Hit
TACCCTGTAGATCCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACTCC548540.12850356041352876RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG548460.12848481923725522RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT545760.12785230453802354RNA PCR Primer, Index 1 (100% over 30bp)
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTCCA539510.12638815014165403RNA PCR Primer, Index 1 (100% over 28bp)
CCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA525970.12321620605735902RNA PCR Primer, Index 1 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTCTCGGGTGCCAAGG520450.12192306489448541Illumina Small RNA Adapter 2 (100% over 21bp)
TAATGTGAATTGCAGGACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA517040.1211242222558262RNA PCR Primer, Index 1 (100% over 32bp)
CCCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAAT509570.1193742649212853RNA PCR Primer, Index 40 (100% over 41bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG502670.11775783846769332RNA PCR Primer, Index 1 (100% over 29bp)
GGCGGATGTGGCGAAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT502530.11772504140921464RNA PCR Primer, Index 1 (97% over 35bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGG489870.11475925026392848Illumina Small RNA Adapter 2 (100% over 21bp)
CCCCCCTCGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCA487240.11414313409393616RNA PCR Primer, Index 1 (97% over 37bp)
CCGCGAGGGGGGCCCGGGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGT480150.11248219734669455RNA PCR Primer, Index 1 (100% over 30bp)
TAATGTGAATTGCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGT479850.11241191793566879RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTCC479250.11227135911361733RNA PCR Primer, Index 1 (100% over 27bp)
TAAGTAAGAGTATTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC474930.11125933559484669RNA PCR Primer, Index 2 (100% over 34bp)
GCGGCTGATGACAGCACTTCTGCTGAGACGCTGTGATTGCTCTGTCCAAAG474230.11109535030245331No Hit
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA470640.11025434001717863RNA PCR Primer, Index 1 (100% over 28bp)
GCGAGGGGGGCCCGGGCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC466000.10916735179331387RNA PCR Primer, Index 1 (100% over 33bp)
GCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAAC464260.1087597312093646No Hit
CACCCGGCCCGGACACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC461420.10809441945165428RNA PCR Primer, Index 2 (100% over 34bp)
GGCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAA459430.1076282326918502No Hit
TCCCTGAGACCCTTTAACCTGTGGTGGAATTCTCGGGTGCCAAGGAACTCC457960.10728386357782409RNA PCR Primer, Index 1 (100% over 27bp)
CCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCA445720.10441646360797394RNA PCR Primer, Index 1 (97% over 37bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG443510.10389873861341768RNA PCR Primer, Index 1 (100% over 29bp)
ACCCTGTAGATCCGAATTTGTGTTGGAATTCTCGGGTGCCAAGGAACTCCA442560.1036761871451695RNA PCR Primer, Index 1 (100% over 28bp)
ACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC439760.10302024597559595RNA PCR Primer, Index 1 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)