FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005398224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005398224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91699093
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC3020307832.93716111237872RNA PCR Primer, Index 1 (100% over 33bp)
AGCGGAGTAGAGCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT60980836.650101762729539RNA PCR Primer, Index 1 (100% over 35bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT57352166.254386834556803RNA PCR Primer, Index 1 (100% over 35bp)
TTAAGTAAGAGTATTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC42850704.672968793704426RNA PCR Primer, Index 1 (100% over 33bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGTATGCC20327042.216711129302009RNA PCR Primer, Index 26 (100% over 51bp)
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGGT16482641.79747034139149No Hit
CTCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA15199881.6575823710709985RNA PCR Primer, Index 1 (100% over 32bp)
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACATG13921091.5181273385114071No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCTTGATGAAAGCACAT7926710.8644262162985625No Hit
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTC7465860.8141694487643406No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTC7398250.8067964205491105No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC6779140.7392810308385493No Hit
GGCGGATGTGGCGAAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT6607450.7205578358337743RNA PCR Primer, Index 1 (100% over 35bp)
CGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA5642820.6153626841216412RNA PCR Primer, Index 1 (100% over 34bp)
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT5075270.5534700326861467No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTCA4226700.46093149470954964No Hit
TCAAGTGATGGAGAGCAATACCCGGGGATGACTGTGACCACATTGGGATGT3776010.4117826988757675No Hit
GACCAATGATGACAAATACCGGCGTATGAGTCTTGGATGATGAATAATACG3479240.3794192380943179No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT3446780.37587939937421194No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG2999230.327073027865172RNA PCR Primer, Index 15 (100% over 36bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT2989520.3260141297144564RNA PCR Primer, Index 1 (100% over 35bp)
TAAGTAAGAGTATTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA2796550.30497030106939005RNA PCR Primer, Index 1 (100% over 34bp)
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCTGATGCCATCACCGC2456060.26783907230140214No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAAC2441340.26623382196375706No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGA2325480.2535990186947651RNA PCR Primer, Index 1 (97% over 37bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA2212420.24126956195739033No Hit
CTCAAGTGATGGAGAGCAATACCCGGGGATGACTGTGACCACATTGGGATG2111650.23028035838915No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA1880370.20505873487756307RNA PCR Primer, Index 1 (100% over 34bp)
GGCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAA1831990.19978278302054744No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA1732280.18890917492499082RNA PCR Primer, Index 1 (100% over 28bp)
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATAC1683350.18357324428497893No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATGA1671310.18226025419902464No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGGA1660020.1810290533626107RNA PCR Primer, Index 1 (100% over 22bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATTCTCGGGTGCCAAGGA1656210.18061356397494577RNA PCR Primer, Index 1 (100% over 22bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA1538830.1678130011602187No Hit
GACCAATGATGAGATTGGAGGGTGTCTGAATCAAAAATTTTGATTAAAGCC1526540.16647274799108427No Hit
GGCCAAGGATGAGAACTCTAATCTGATTTTATGTGCTTCTGCTGTGATGGA1516730.1654029446070966No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCTC1435490.1565435330968868No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1423740.15526216818742145RNA PCR Primer, Index 1 (100% over 32bp)
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTCT1379270.15041261095134278No Hit
ACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGA1371020.14951292920639903RNA PCR Primer, Index 1 (97% over 37bp)
GGTCCAGGATGAAACCTAATTTGAGTGGACATCCATGGATGAGAAATGCGG1363360.14867758833776035No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGGAA1340790.14621627718825964RNA PCR Primer, Index 1 (100% over 23bp)
TGGCCAAGGATGAGAACTCTAATCTGATTTTATGTGCTTCTGCTGTGATGG1329130.14494472698873911No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG1186170.1293546055030228RNA PCR Primer, Index 1 (100% over 29bp)
CTGCGATGATGGCATTTCTTAGGACACCTTTGGATTAATAATGAAAACAAC1159470.12644290821938664No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGATT1141050.12443416425067584No Hit
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG1140470.12437091389769798No Hit
TTCCTATGATGAGGACCTTTTCACAGACCTGTACTGAGCTCCGTGAGGATA1084840.11830433262845905No Hit
CGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAG1081580.11794882202379034RNA PCR Primer, Index 26 (100% over 38bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG1074660.1171941798813648RNA PCR Primer, Index 15 (100% over 36bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC1065950.11624433406336963RNA PCR Primer, Index 1 (100% over 27bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC1002240.1092966099457494RNA PCR Primer, Index 1 (100% over 26bp)
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCTC953550.10398685186559042No Hit
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG936880.10216894947914043RNA PCR Primer, Index 1 (100% over 29bp)
GGAACAGTACTCAAGTGGCCGAAGAGGACACGTTGGAATTCTCGGGTGCCA929560.10137068640362669No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGGAG6421800.044.7665861
CGGAGTA6429950.044.7483
GCGGAGT6431900.044.7406732
GGAGTAG6435650.044.7315524
GAGTAGA6437100.044.7173045
AGTAGAG6437900.044.7081836
GAGCAGT6437150.044.70363610
GTCTACG339350.044.7005841
CACAGAT768100.044.5977331
GCGAAGT703600.044.54211411
TGGCGAA703400.044.538579
GCAGTTG6463000.044.52794312
AGCAGTT6467500.044.49420511
CAGTTGG6478750.044.4450513
AGAGCAG6481750.044.4156049
TTAAGTA4527250.044.299141
GGATGTG708950.044.2474174
TGTGGCG711300.044.0945367
ACCCGTA291850.044.091652
ATGTGGC711900.044.0788466
Produced by FastQC (version 0.11.5)