FastQCFastQC Report
Thu 9 May 2024
EGAF00005398224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005398224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91699093
Total Bases4.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA3021781732.95323433569839RNA PCR Primer, Index 1 (100% over 32bp)
AGCGGAGTAGAGCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA60991186.651230454373197RNA PCR Primer, Index 1 (100% over 34bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA57373786.25674454599022RNA PCR Primer, Index 1 (100% over 34bp)
TTAAGTAAGAGTATTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA42875394.675661295799294RNA PCR Primer, Index 1 (100% over 32bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGTATGC20334792.217556284880593RNA PCR Primer, Index 26 (100% over 50bp)
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGG16495491.798871663866948No Hit
CTCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC15219711.6597448788288454RNA PCR Primer, Index 1 (100% over 31bp)
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT13930541.5191578830556154No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCTTGATGAAAGCACA7929500.8647304723068526No Hit
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACT7468620.814470433202649No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGT7404060.8074300146022164No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC6784440.7398590082019677No Hit
GGCGGATGTGGCGAAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA6608970.7207235953795094RNA PCR Primer, Index 1 (100% over 34bp)
CGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC5647780.6159035836919347RNA PCR Primer, Index 1 (100% over 33bp)
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC5080000.5539858502199144No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTC4230720.4613698850870858No Hit
TCAAGTGATGGAGAGCAATACCCGGGGATGACTGTGACCACATTGGGATG3777850.41198335516797313No Hit
GACCAATGATGACAAATACCGGCGTATGAGTCTTGGATGATGAATAATAC3480250.37952938095036554No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC3448580.3760756935731087No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT3005200.3277240702915131RNA PCR Primer, Index 1 (100% over 35bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA2990200.3260882853007063RNA PCR Primer, Index 1 (100% over 34bp)
TAAGTAAGAGTATTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2798320.3051633236983053RNA PCR Primer, Index 1 (100% over 33bp)
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA2705760.2950694397816999No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCTGATGCCATCACCG2456980.26793940044750497No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG2327370.25380512760360674RNA PCR Primer, Index 15 (100% over 36bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC2215280.24158145162897085No Hit
CTCAAGTGATGGAGAGCAATACCCGGGGATGACTGTGACCACATTGGGAT2112180.2303381561254919No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1882040.20524085227320624RNA PCR Primer, Index 1 (100% over 33bp)
GGCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGA1832480.19983621866358045No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC1735920.18930612541609326RNA PCR Primer, Index 1 (100% over 27bp)
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA1684750.18372591755078754No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG1672740.1824161990348149No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGG1660930.1811282909853863Illumina Small RNA Adapter 2 (100% over 21bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATTCTCGGGTGCCAAGG1657320.18073461206426544Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA1541010.16805073524554925No Hit
GACCAATGATGAGATTGGAGGGTGTCTGAATCAAAAATTTTGATTAAAGC1527410.16656762352055107No Hit
GGCCAAGGATGAGAACTCTAATCTGATTTTATGTGCTTCTGCTGTGATGG1518070.165549074732942No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT1437130.15672237892254834No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1426040.15551298855267848RNA PCR Primer, Index 1 (100% over 31bp)
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTC1379770.15046713711770301No Hit
ACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG1372020.14962198153911946RNA PCR Primer, Index 15 (100% over 36bp)
GGTCCAGGATGAAACCTAATTTGAGTGGACATCCATGGATGAGAAATGCG1363580.14870157985095883No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGGA1342800.14643547237702775RNA PCR Primer, Index 1 (100% over 22bp)
TGGCCAAGGATGAGAACTCTAATCTGATTTTATGTGCTTCTGCTGTGATG1329490.14498398582851849No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA1186940.12943857579921755RNA PCR Primer, Index 1 (100% over 28bp)
CTGCGATGATGGCATTTCTTAGGACACCTTTGGATTAATAATGAAAACAA1159910.12649089124578364No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT1141350.124466879950492No Hit
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG1140970.12442544006405822No Hit
TTCCTATGATGAGGACCTTTTCACAGACCTGTACTGAGCTCCGTGAGGAT1086330.11846682060421253No Hit
CGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGA1081690.11796081778038961RNA PCR Primer, Index 26 (100% over 37bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT1075730.11731086587737569RNA PCR Primer, Index 1 (100% over 35bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC1066920.11635011482610848RNA PCR Primer, Index 1 (100% over 26bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT1002630.10933914035551039RNA PCR Primer, Index 1 (100% over 25bp)
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT954150.1040522832652227No Hit
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCA937400.10222565669215508RNA PCR Primer, Index 1 (100% over 28bp)
GGAACAGTACTCAAGTGGCCGAAGAGGACACGTTGGAATTCTCGGGTGCC930640.10148846292296479No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)