FastQCFastQC Report
Thu 16 Jun 2022
EGAF00005398970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005398970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82532572
Sequences flagged as poor quality0
Sequence length90
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT12585491.5249118856976855TruSeq Adapter, Index 13 (97% over 40bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1016910.12321317212796908TruSeq Adapter, Index 13 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)