FastQCFastQC Report
Sat 2 Jul 2022
EGAF00005398974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005398974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70311491
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC63419649.019811569633761TruSeq Adapter, Index 5 (100% over 50bp)
GCTCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC2756850.39209095992573956TruSeq Adapter, Index 5 (98% over 50bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1598370.22732699552623623TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACACAGTGATCTCGTATGC1214040.1726659444613399TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACACAGTGATCTCGTATGC1132950.1611329789607221TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACACAGTGATCTCGTATGC1107020.157445103816672TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1105240.15719194462822586TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACACAGTGATCTCGTATGC990810.1409172221934534TruSeq Adapter, Index 5 (98% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC981190.13954902478173875TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACACAGTGATCTCGTATGC975580.13875114666534377TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAGTGATCTCGTATGC953930.13567199136766991TruSeq Adapter, Index 5 (98% over 50bp)
GTTCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC833770.11858232390492189TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC815910.11604219856466988TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC738130.1049799953751514TruSeq Adapter, Index 5 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)